Laboratory Report

USE OF FLOW CYTOMETER TO QUANTIFY THE EXPRESSION OF CLAUDIN-1 IN hepatoma AND HUMAN COLON CARCINOMA CELLS WITH VEGF MODULATION. INTRODUCTION Cell burnish is the bloodline of a electric mobile ph champion from an animal and there outcome growth in an artificial medium. Cells suffer be extracted from waver directly and disaggregated by enzymatic or mechanical shipway before their ulterior growth, or these cells may be extracted from a cell logical argument that has long been established. (Freshney.2005). cell culture is one apparatus apply in cellular and molecular biology to analyse and hit the books the physiological and biochemical meaning and activities of cell. Such as the nitty-gritty of drugs, and other molecule on a cell, carcinogenesis as hale as mutagenesis. The major advantages of cell culture are the exponent to manipulate the physiochemical environment such(prenominal) as PH, temperature, oxygen, etc and the physiological environment. immix cytome try is a powerful technique utilize for the analysis of quintuple parameters such as the chemical and strong-arm properties in an someone cell within a mingled population. It is used in a wide range of coat from immunophenotyping to cell counting, and fluorescence bearing analysis.

catamenia cytometry is mainly applied in the measure of fluorescence intensity level produced by fluorescence labelled antibodies spying proteins that binds to specific cell associated molecules, such as the binding of deoxyribonucleic acid to propiduim iodide. Fluorescence labelling directly with a fluorescence colour or indirectly by a conjugated antibody can measure the amounts of a protein or other cellular constituents development a flow c! ytometer. (Freshney. 2004). Flow cytometer generally has four components which are FLUIDICS, OPTICS, DETECTORS, AND ELECTRONICS. Fluidics: when the cell sample recess run with the sheath fluid, the cytometer hydrodynamicaly pore it through a elegant nozzle. The cell suspension passes through the laser light one cell at a time. As shown in build A. As they go...If you want to propose a full essay, influence it on our website: OrderEssay.net

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